Rbf Promotes Biofilm Formation by Staphylococcus aureus via Repression of icaR , a Negative Regulator of icaADBC
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چکیده
منابع مشابه
RsbU-dependent regulation of Staphylococcus epidermidis biofilm formation is mediated via the alternative sigma factor sigmaB by repression of the negative regulator gene icaR.
Transposon mutagenesis of rsbU leads to a biofilm-negative phenotype in Staphylococcus epidermidis. However, the pathway of this regulatory mechanism was unknown. To investigate the role of RsbU in the regulation of the alternative sigma factor sigma(B) and biofilm formation, we generated different mutants of the sigma(B) operon in S. epidermidis strains 1457 and 8400. The genes rsbU, rsbV, rsb...
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We read with interest the article of Arciola et al. (1) on the presence of icaA and icaD genes in Staphylococcus aureus and S. epidermidis isolates from catheter-associated infections and its correlation with a slime-positive phenotype detected with Congo red agar. These are the organisms most frequently the cause of foreign-body-related infections. The polysaccharide intercellular adhesin (PIA...
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The proteins encoded by the intercellular adhesin genes (icaADBC) synthesize the polysaccharide poly-N-acetylglucosamine (PNAG), which contributes to the formation of a biofilm by Staphylococcus aureus. In addition to the proteins that synthesize PNAG, the ica locus also encodes the TetR family transcriptional regulator IcaR (23). The icaR gene is transcribed divergently from icaADBC (10) and i...
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Both Staphylococcus aureus and S. epidermidis are capable of forming biofilm on biomaterials. We used Tn917 mutagenesis to identify a gene, rbf, affecting biofilm formation in S. aureus NCTC8325-4. Sequencing revealed that Rbf contained a consensus region signature of the AraC/XylS family of regulators, suggesting that Rbf is a transcriptional regulator. Insertional duplication inactivation of ...
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Objective(s):Staphylococcus aureus is a Gram-positive pathogen, well known for its resistance andversatile lifestyle. Under unfavourable conditions, it adapts biofilm mode of growth. For staphylococcal biofilm formation, production of extracellular polymeric substances (EPS) is a pre-requisite, which is regulated by ica operon-encoded enzymes. This study was designed to know the impact of ascor...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2009
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00913-09